Sight Search

Fluorescence Imaging help Identify Coral Bleaching

The nearinfrared (NIR) fluorescence imaging system the authors call “the beast” includes a
camera, housing, tripod, a longpass (LP) near-infrared filter, tricolor or blue LED dive lights, shortpass (SP) light filters and a dual diopter holder for the filters. Divers can use a much
simpler setup to help with NIR imaging.
Text by Jittiwat Sermsripong, Latisha Jefferies, Abby Jennings, Andrea Giordano and Barry W. Hicks | Photos by Barry W. Hicks

We live in the Anthropocene. Humans affect the very world we inhabit. Deforestation, fossil fuel use and environmental pollutants such as plastics and pesticides are changing our world. The oceans and the reefs within them are not immune to these effects. Coral-bleaching events and the coral death that often follows are among the most serious challenges in marine ecology today. Despite decades of scientific investigation, researchers have not yet discovered an effective way to identify the onset of bleaching events, which result in the loss of the endosymbiotic algae that reside within corals. The algae contain chlorophyll and use photosynthesis to produce carbohydrates that they supply to the corals.

Without the algae, there’s no chlorophyll for the corals. Researchers can monitor changes in terrestrial chlorophyll by examining near-infrared chlorophyll fluorescence (NIRChlF), even by satellite, but they have done little work on near-infrared (NIR) imaging underwater. The first 6 feet of the ocean’s surface absorbs most NIR light from the sun, so aerial or satellite imaging
of NIRChlF isn’t possible.

We recently published a scientific paper using commercially available equipment to image NIR fluorescence from chlorophyll in corals.1 We lack the time and ocean access, however, to do much of the basic control work that might allow remote underwater imaging of NIRChlF to become a part of the solution to identifying bleaching events. We invite divers and underwater photographers who are willing to make a modest investment in their imaging equipment to help us further develop and improve our methodology. Any diver can become a part of the solution by engaging in citizen-science research. We affectionately call the camera setup we used to get data for our published work “the beast.” 
Not all the equipment is necessary to begin, but an understanding of how we have assembled our NIR fluorescence-imaging system should help others to mimic and improve upon it.
You must have a camera with custom white-balance settings and an underwater housing. You will need to remove the internal infrared filter, which you can do yourself if you are a camera expert or you can pay a commercial dealer about $250 to make the modification for you.

You will also need a longpass NIR filter (about $15) to put over the lens. If you want to get more serious, a pair of good tricolor or blue LED dive lights ($150–$1,000), hardware and tripod ($100–$400), shortpass light filters ($250) and a dual diopter holder for the filters ($250) will allow you to make your own similar beast setup.

To illustrate the process, we collected images of a great star coral (Montastraea cavernosa) on a night dive in the Caribbean Sea near Carriacou, Grenada. We used a camera with a macro lens on a tripod to take these images, but neither the tripod nor macro lens is an absolute necessity.
For the far-left image we used white-light illumination and no filter. The middle image is with blue-light illumination and a yellow filter for viewing green fluorescent proteins and red emission (mostly from chlorophyll). The right image is with blue LED or white illumination below 675 nanometers (nm) and an IR 720 nm filter on the camera showing only the NIRChlF.

The center image clearly shows that fluorescent proteins and chlorophyll are not identically distributed, which may explain why fluorescent proteins don’t prevent solar induced bleaching in the native reefs despite research indicating that they can act as a sunscreen. If this sunscreen is supposed to help protect the endosymbiotic algae inside the corals, it is not sufficiently applied to do the job. The NIRChlF image on the right confirms the chlorophyll distribution. Regions in the center image showing the most intense red fluorescence are also the  brightest in the NIRChF image.

Divers can perform this type of fluorescence imaging in daylight because of limited infrared light penetration below about 10 feet. To start your own imaging sequence, find a coral near a sandy bottom where the tripod can be set up to minimize impact. Use a white dive slate as a target for setting custom white balances on the camera; generate a separate setting to use each with each combination of lighting and filter set (white lighting with no filter, blue LED lighting with yellow filter, and blue lighting with a NIR 720 nm longpass filter). Using a tripod will ensure that all the images will be in register. A standard lens at a greater distance rather than a
macro lens might capture more information on larger specimens and without a narrow depth of focus, but it could require longer exposures and necessitate use of a tripod to prevent blurred images. A macro lens is desirable because it produces a greater level of fine structure onchlorophyll distributions, and the close working distance between camera and subject means the water absorbs less NIR light. As with all macro photography, issues such as narrow focal depth can be problematic, but once you get started you’ll be hooked.
This imaging sequence of a great star coral uses, from far left, white lighting with no filter, blue LED lighting with a yellow filter, and blue lighting with a near-infrared 720 nm longpass filter. The center image shows green fluorescent proteins and red emissions from chlorophyll. The image on the right shows near-infrared chlorophyll fluorescence.

We want to improve this imaging methodology so autonomous underwater vehicles (AUVs) can use it for remote imaging on large sections of the reef, similar to satellite imaging for terrestrial NIRChlF. While there are no guarantees, such technology might be able to identify the onset of a bleaching event. Besides the unique photos you get from underwater NIRChlF imaging, NIR fluorescence-imaging equipment can help with other research.

With assistance from the dive community, we would like to investigate the following questions:
• How deep can we photograph NIRChlF emission in daylight without external illumination?
• How deep can we find organisms that have endosymbionts containing chlorophyll?
• How far from specimens can we photograph NIRChlF  emission underwater with either ambient solar illumination (in daylight) or external LED illumination (at night)?
• What lenses work best at the maximum distance? 
• Which sponges have algal endosymbionts?
• What unexpected marine organisms (such as tunicates or bivalves) have chlorophyll-containing endosymbionts?
• Which organisms that normally contain chlorophyll (such as gorgonians) have members that do not?
• How much does chlorophyll content change seasonally in one coral?
• How much NIR does endolithic algae in bleached or diseased corals emit?
• Using video and time-lapse analysis, does chlorophyll redistribute when illuminated? Can this be detected? On what time scale? How does this vary with species?
• Can chlorophyll photobleaching in one polyp be overcome by donation of chlorophyll from a neighboring polyp?
• Art can motivate some people in a way that science cannot. Altering white balance, lighting and filters when performing underwater NIR fluorescence imaging can lead to color effects like those seen in terrestrial NIR images. What types of art can you produce to motivate others to pursue underwater NIR fluorescence imaging?

There clearly is much to be done. If you are interested in helping to conduct some of these experiments and would like more information, contact Barry Hicks at barry.hicks@afacademy.af.edu.

REFERENCE

1. Oh T, Sermsripong J, Hicks BW. Using scuba for in situ determination of chlorophyll distributions in corals by underwater near-infrared fluorescence imaging. J. Mar.
Sci. Eng. 2020; 8(1):53.

Categories

 2023
immersion and bubble formation 232bar 24Hours AGE AIDA Accident management Accidents Acid reflux Acute ailments Adam Sokolski Advanced courses Rescue diver Aerobic exercise After anaesthesia Aged divers Air Ambulance Air Quality Air consumption Air exchange centre Air hose failure Air supply Airway control Air Alert Diver December 2022 Alert Diver Magazine Alert Diver March 2023 Algorithms Alice Cattaneo Alice Modolo Alopecia Alternate Airsources use Alternater Air Source Alternative gas mix Altitude changes Altitude diving Altitude sickness Aluminium Oxide Ama divers Amino acids Amos Nachoum Anaerobic Metabolism Anilao Animal intelligence Animal life Annual renewal Antarctica Anxiety Apea Apex predators Apnea addicts Apnea Apnoea Aqua corps Aquatic creatures Aquatic life Aquatics and Scuba Diving Archaeology Argonaut octopus Argonauts Argon Arrythmia Arterial Gas Embolisms Arterial gas embolism Arthroscopic surgery Aspirin Audible signals Aurel hygiene Australian Flat backed Australia Aviation o2 BCD BHP BLS BWARF Baacterial infections Back adjustment Back pain Back treatment Backextensors Backmount CCR Badages Bag valve mask Bags Bahamas Bail out cylinder Balancing Bandaids Barbell back squat Barometric pressure Barotrauma Basic Life Support Basslets Batteries Beach entry Beached coral Becky Kaga Schott Becky Kagan Schott Beluga whales Bench press Bends Benign prostate hyperplasia Benzophenones Beth Neale Beyond Standards Big Sur Bilikiki Tours Biophysics Black Water Photography Black Blood flow Blood pressure Blood thinners Blue Desert Blue Wilderness Blue economy Blue heron Bridge Bluff Blurred vision Boat diving Boat etiquette Boat safety Boats Bobbit worm Boesmans gat Boesmansgat Bonaire Bone fractures Bouyancy compensators Bouyancy control device Bouyancy controls Boyle's Law Boyle\'s Law Bradycardia Brain Brandon Cole Breast Cancer Breath Hold Diving Breath hold diver Breath holding Breath hold Breath-hold Breathing Gas Breathing gas contamination Breathing oxygen Breathing Breathold diving Bright Bank Broken bones Bruising Bubble detection Bubbleformation Buddy Exercise Buddy checks Buoyancy Burn wounds Burnshield Burns Business B CCR CE markings CGASA CMAS CNS CO2 COVID-19 Updates COVID-19 COVID CPR CSI Cabin pressure Caissons diseas California Camera equipment Camera settings Cameras Cancer Remission Cancer treatments Cancer Cannabis and diving Cannabis Cape Diver Research Cape Nudibranchs Cape Town Dive Festival Cape Town Dive Sites Cape Town CapeTown Carbon Monoxide Carbon dioxide Cardiac Health Cardiac research Cardiaccompromise Cardio health Cardiological Cardiomyopathy Caribbean Carmel Bay Carribean Conservation Catalina Island Cave Cave diving Cave divers Cave diving Cave exploration Caves Cave Cenotes Ceotes Challenging Environments Chamber Locations Chamber Safety Chamber maintenance Chamber medical staff Chamber science Chamber treatment Chamber Charging batteries Charles' Law Charles\' Law Charles\\\' Law Charles\\\\\\\' Law Charles\\\\\\\\\\\\\\\' Law Charlie Warland Chemotherapy Chest compressions Children diving Chiropractic Chlorophll Chokka Run Christina Mittermeier Chuck Davis Chuuk lagoon Citizen Conservation Citizen sciences Citizen science Clean Air Cleaning products Cleeve Robertson Climate change Closed Circuit Rebreathers Closed Circuit Rebreather Clothing Cmmunity partnership Coastal diving Coastalexcursion Cold Water Cold care Cold sores ColdWater Cold Commercial Fishing Commercial diving Commercial operations Commercial schools Common consideration Common understanding Communication Compact Cameras Composition Compressed Air Compressed gas Compressor operators Compressors Concussion Congestive heart Faiture Consercation Conservation Photographer Conservation photography Conservation Contact lenses Contaminants Contaminated air Coping with cold Coral Bleaching event Coral Conservation Coral Reefs Coral Restoration Coral bleaching Coral preservation CoralGroupers Corals Core strength Corona virus Coro Coservation Costamed Chamber Courtactions Cozumel Cradiac valvular Crime scene Cristina Mittermeier Crohns disease Crowns Crystal build up Crystallizing hoses Cubs Cutaneous decompression Cutting tools Cylinder Ruptures Cylinder capacity Cylinder handwheel Cylinder safety Cylinder valves Cylinder weight Cylinders DAN Courses DAN Europe DAN Profile DAN Researchers DAN hotline service DAN insure DAN medics DAN members DAN report DANTraining DCI DCS Decompressions sickness DCS theories DCS DEMP DM training DNA DPV DReams DSMB Daan verhoeven Dahab Dalton's Law Dalton\'s Law Dalton\\\'s Law Dalton\\\\\\\'s Law Dalton\\\\\\\\\\\\\\\'s Law Danel Wenzel Dangerous Marinelife Dauin island Dave McCowan David Doubilet Dean's Blue Hole Dean\'s Blue Hole Deco dives Decompression Illness Decompression Sickness Decompression Stress Decompression benenfits Decompression chamber Decompression illsnes Decompression ilness Decompression limits Decompression treatment Decompression Decomression sickness Decorator crabs Deep Freediving Deep diving Deep water exploration Deepest SCUBA Dive Delayed Offgassing Dennis Guichard Dental Depth limits Dever Health Diadema Response Team Diagnosis Diaphragms Diets Diopter Dirty water Discomfort Diseases Disinfection Disorientation Distraction Dive Accident Dive Action Dive Buddy Dive Chamber Dive Chmber Dive Computer Dive Destinations Dive Destination Dive Fitness # fit to dive @Dive health Dive H Dive Industry Dive Instruction Dive Instructor Dive Lights Dive Masters Dive Medical Form Dive Medical Dive Practices Dive Professionals Dive Pros Dive Rescue Dive Research Dive Safari Dive Safety Tips Dive South Africa Dive Taiwan Dive Training Dive Travel Wakatobi Dive Travel Dive accidents Dive bell Dive buddies Dive caves Dive centre rules Dive communications Dive computers Dive courses Dive cover Dive cylinder Aluminium Dive equipment Dive excursions Dive exercise Dive exeriences Dive experience Dive fitness Dive gear Dive heallth Dive health Dive in Africa Dive insurance Dive leaders Dive masks Dive medical insurance Dive medicals Dive medicines Dive medicine Dive operators Dive opportunities Dive planning Dive procedures Dive safety 101 Dive safety briefing Dive safety Dive safe Dive skills Dive staff Dive teams Dive travels DiveLIVE DiveTravel Diveleader training Diveleaders Divelights Diver Ethisc Diver Food Diver Health Diver Profile Diver Travel Diver education Diver infliencers Diver on surface Diver recall Diverover 50 Divers Alert Diversafety Divers Divesites Diving Divas Diving Equipment Diving Family Diving Fatalities Diving Feet Diving Helmets Diving Kids Diving Programs Diving Trauma Diving career Diving emergencies Diving emergency management Diving etiquette Diving fit Diving guidelines Diving history Diving injuries Diving science Diving suspended Diving Dizziness Dizzyness Dolphins Domestic Donating Blood Donation Doug Perrine Dowels Dr Rob Schneider Drift diving Drysuit diving Drysuit valves Drysuits Durban Dynamic environment Dyperbaric medicines EAPs EAP EKG EMS EN standards Ear barotrauma Ear pressure Ear wax Ears injuries Eat fish Eco friendly Ecowise Education Electronic Embolisms Emergency Action Plan Emergency Planning Emergency action planning Emergency decompression Emergency plans Emergency treatments Emergency underwater Oxygen Recompression Emergency Endurance Entry Envenomations Enviromental Protection Environmental factors Environmental impact Environmental managment Environmental stewardship Equalisation Equalization Equipment care Equipment failure Equipment inspection Equipment significance Evacuations Evacuation Evaluations Even Breath Evironmetaly friendly Exercise Exercising Exhaustion Exploration dives Exposure Protection Extended divetime Extinction Extinguisher Extreme treatments Eye injuries Eye protection FAQ Face computer Factor V Leiden Failures FalseBay Diving Fatigue Faulty equipment Feet Femal diver Female divers Fenivir Fetus development Field practice Filling stations Fillings Fin Foot Fins Fire Coral Fire Safety Fire extinguisher Firefighting Fires First Aid Equipment First Aid Kit First Aid Trainig First Aid Training First Aid kits First Aid Fish Identification Fish Life Fishing Fish Fit to dive Fitness Levels Fitness Training Fitness evaluation Fitness to dive Fitnesstrainng Fitness Flying Focus lights Food Footissues Foundations Fractures Francesca Diaco Francois Burman Fred Buyle Fredive Free Student cover Free diving Free flow Freedive Competition Rules Freedive Competiton Freedive INstructor Freedive Safety Freedive Training Freedive computer Freedive modes Freedive rescue procedures Freediver Staff Instructor Freediver Freedive Freediving Instructors Freediving performance Freediving Fur rade Galapagos Gar Waterman Gardens of The Queen Gas Density Gas Planning Gas consumption Gas emboli Gas laws Gas management Gas mixes GasPerformance Gasblends Gases Gass bubbles Gastoeusophagus Gastric bypass Gastroenterologist Gas Gear Servicing Germs Geyer Bank Giant Kelp Forest Giant Kelp Giant stride Girls that Scba Gobies Golden fish Gordon Hiles Great White Sharks Green sea turtle Green turles Greenlings Guinness World Record Gutt irritations HBOT HBO HCV HELP HIRA HMLI HMS Britanica Haemorhoid treatment Hand signals Harry Chammas Havanna Hawaii Hawksbill Hazard Description Hazardous Marine life Hazardous marinelife Head injuries Headaches Health practitioner Healthy Food Heart Attack Heart Health Heart Rate monitor Heart fitness Heart rates Heart rate Heart Heat loss Heat stress Helen Walne Heliox Helium Gas Helium Hematoma Hemodynamic Hepatitis C Hepatitus B Hiatal Hernia High Pressure vessels High pressure hoses High temperatures Hip strength Hip surgery Hippocampus History Hole in the heart Hood Hot Human diver Humans Hydrate Hydration Hydrogen Hydroids Hydrostatic pressure Hygiene Hyperbaric Chamber Hyperbaric Oxygen Treatments Hyperbaric Oxygen Hyperbaric research Hyperbaric treatment Hyperbarics Hypertension Hypothermia Hypoxia I-52 found INclusivity IdentiFin Imaging Immersion Immine systems In Water Recompression Increased pressure Indemnity form Indian Ocean Indigo SCuba Indonesia Inert gas Infections Infra red Imaging Inhaca Ocean Alliance Injections Inner ear Instinct Instruction Instructors Insulation Insurance policy Insurance Integrated Physiology International travel International Internship programs Internship Interval training Investigations Irritation Irukandji Syndrome Isotta housing Italy Itchy Rash Its OK Jellyfish Jennifer Hayes Jill Heinerth Joanna Wyrebek John Vowles Joint pain Joshua Journal for Technical Diving Junior Open Water Diver KZN South Coast KZN Karen van den Oever Kate Jonker KateJonker Kelp Forest Kelp forests Kenya Kidneys Kids scubadiver Komati Springs KwaZulu Natal Kwazulu-Natal LED lights LED Labour laws Lake Huron Lara Lambiase Laryngospasm Lauren Arthur Leanne Walmsley Learning to dive Leatherbacks Leatherback Legal Network Legal advice Legislation Lembeh Straights Lenses Leslie Lwaney Leukemis Liability Insurance Liability Risks Liability releases Liability Life expectancy Lifestyle Lighting equipment Lighting Lightroom editing Lights Lionfish Live aboard diving Liveaboard Liver Toxicity Liver diseas Liz Louw Loss of consciousness Lost at sea Lost divers Low Visability Low blood platelets Low blood pressure Low pressure deterioration Low volume masks Lumpsuckers Lung Irritation Lung flexibility Lung function Lung injuries Lung over expansion Lung squeeze Lung surgery Lung MOD MOzambique diving MPA Macro photography Mafia Island Maintenance Malaria Mammalian Dive Response Mammalian effect Manatees Mandarin Fish Marfan syndrome Marine Biologist Marine Biology Marine Mega fauna Marine Science Marine Scientists Marine conservation Marine parks Marine plants Marinelife Marinescience Markus Dirschi Marlin Marne protecte areas Mask clearing Masks Mask Master scuba diver Matty Smith Maximum operating depth Medical FAQ Medical Q Medical emergencies Medical forms Medical oxygen Medical questionaire Medical statement Medical team Medicalquestionaires Medicalresearch Medicalstudents Medication Mehgan Heaney-Grier Membership benefits Menopause Menstruation Mental health Mermaid Danii Mesophotic Metotrexate Mexico Michael Aw Micro Photography Microbubbles Middle ear pressure Mike Bartick Military front press Military Misool Resort Raja Ampat Mixed Gas Mono Fins Mooring lines More pressure Motion sickness Motionsickness Mount Kilimanjaro Mozambique Muck Diving Muscle pain Mycobacterium marinum NDL limits NSRI Narcosis National Geographic Nature Nausea Nauticam Ambassadors Nauticam Ambassador Nautilus Navigation Ndibranchs Neck pain Neoprene layers Neuro assessments Neurocognitive research Neurological assessments Neuromotor exercises New Caledonia Nichola Bird Nicorette Nicotine Nitrogen Narcosis Nitrogen build up Nitrox No Decompression Limits No-decompression limits No-decompression Non-nano zinc oxide Non-rebreather Mask Non-smoking Nondiving related illness Nonrebreather masks Normal Air North Sulawesi Northern Taiwan Nosebleeds Nudibranchs Nuno Gomes Nutrition O2 enriched O2 oxygen provider. O2 providers O2 servicing O2 treatments O2treatments OOxygen maintenance Ocean Alliance Ocean Projects Ocean Research Ocean animals Ocean clean up Ocean community Ocean conservation Ocean life Ocean mammals Ocean migrations Ocean pollution Oceangate Ocean Octopus Oil contamination Olive Ridley Open Ocean Open water divers Operatorethics Optical focus Oral contraseptives Orbital implants Oronasal mask Osteonecrosis Otters Out and about Out of air Outer ears Outreach Overhead Envirenments Oxygen Administration Oxygen Cylinder Oxygen Toxicity Oxygen Units Oxygen deficit Oxygen deicit Oxygen dificiency Oxygen ears Oxygen equipment Oxygen explosions Oxygen kit Oxygen masks Oxygen providers Oxygen safety Oxygen supplies Oxygen supply Oxygen systems Oxygen therapy Oxygen treatment Oxygen P J Prinsloo PADI Freedivers PFI PFOs PFO PJP Tech PTSD Paper Nautilus Paralysis Parentalsupervision Part 3 Partner Training Patent foramen ovale PatentForamen Ovale Pemba Island Peri-peri Divers Personal Perspective Petar Denoble Peter Lindholm Philippine Islands Philippines Phillipines Photographers Photographer Photography tips Photography Physical Fitness Physioball Physiology Physiotherapy Pills Pilot Whale Pistons Planning Plastic pollution Plastic Plimsoll Interface Pneumonia Pneumothorax Poison Pole fishing Polka Dot Bat fish Pollution Pool Diving Pool chemicals Pool maintenance Pool workout Pools Post traumatic Post-dive Potuguese man-of-war Pre-dive fitness Pre-dive Predive check Pregnancy Pregnant divers Preparation Prepared diver Press Release Preventions ProDive Port Elizabeth Product scuba nudi Professional rights Protection Provider course Psycological Pulmanologist Pulmonary Barotrauma Pulmonary Bleb Pulmonary Edema Pulmonary Hypertension Pulse Punture wounds Pure Air Pure Apnea Purge Quit Smoking Q RAID South Africa RCAP REEF RMV ROS Radio communications Range of motion Rashes Reactive oxygen species Rebreather diving Rebreatherdive Recces Rechargeable batteries. Rechargeable Recompression chamber Recompression treatment Recompression Recreational dives Recycle Red SEA Reef Chcek Reef Conservation Reef Environmental Education Foundation Reef protection Reef safe Reef surveyors Refractive correction Regal Sea Goddesses Regulator failure Regulators Regulator Remote areas Remote dive locations Remote islands Renewable Rental gear Report incidents Rescue Divers Rescue Procedure Rescue breathing Rescue breaths Rescue diver Rescue skills Rescue skill Rescue training Rescue Researcher profile Research Respitory Minute Volume Resume diving Return To Diving Return to diving Reuseable items Rhinopias Risk Assessments Risk assesments Risk assessment Risk elements Risk management Risk mitigation Risks of Seizures River diving Riviera Maya Roatan Marine Park Roatan Ronblom Rubber ducks SABS 019 SAC SMB SRC SafariLive Safe diving practices Safety Concerns Safety Gear Safety Stop Safety in Air Safety SaherSafe Barrier Salisbury Island Salish Seas Salty Wanderer Sanitising Sanne Volja Sara Andreotti Sara Banderby Sara Campbell Sardine Run Sargassum sea Saturation Diving Saturation diver Save our seas Schrimps Science of diving Science Scoliosis Scombroid Poisoning Scorpion Fish Scuba Air Quality Scuba Guru Scuba Injury Scuba Instructor Scuba children Scuba divers Scuba dive Scuba diving Scuba education Scuba gear Scuba health Scubalearners Scubalife Sea Horses Sea Turtles Sea rescue Sea slugs Seagrass Sealcolonies Sealife Seals Seasickness Seaweeds Seaweed Sea Self Rescue Send Nudi Shallow Water Blackout Shallow dives Shark Protection Shark Research Shark conservation Shark diving Sharks Shipwrecks Shit Happens Shore entries Shoulder strength Sidemount Sideplank Signalling devices Signs and Symptoms Silty bottoms Sit-ups Sixgill Sharks Skills in action Skin Bends Skin outbreak Skin rash Smart phone photography Snells Window Snorkeling Snorkels Social Distancing Sodwana Bay Solomon Islands Sonnier bank South Africa Southern Sea Otters SpareAir Special Operations Special forces units Sperm Whales Spinal Bend Spinal bends Spinal cord DCS Spinal dura Spinal pain Spinner dolphins Spleen Splits Sports medicine Squeezes Squid Run Stability exercise Stage cylinder Standars Statin Mediction Stay Fit Stay Warm Steel Stefan Randig Stents Step ups Stephen Frink Stepping up Stobes Stockton Rush Stonetown Stretch band exercise Stretch bands Stretching Strobe Lighting Strobes Stroke Submerge tech Submerged Sudafed Sulawesi Sun protection Sun screen Sunscreen Supplemental oxygen Surface Air Consumption Surface Consumption Rate Surface Marker Buoys Surface supplied Air Surfaced Surfers Surgeries Surgery Survivor Suspension training Swim Fitness Swimmers health SwimmingIn wateractivities Swimming Sylvia Earl Symbiosis Symbiosys TRavel safety Tabata protocol Talya Davidoff Tank valve Tanzania Tara Panton Tattoes Tchnical diving Team Awareness Tec Clark Tec divers Tec diving Tech diving Technical Diving Technical diver Technical divng Temperature Homeostatis The Bends The Cavettes The Produce The Titanic Wreck The Wild Coast The greatest Shoal The silent world The truth Thermal Notions Thermoregulation Thomas Peschak Thresher shark Thunder Bay National Marine Sanctuary Tides Tips and trick Tonga Tooth squeeze Transplants Trashbag Trauma Travel Safe Travel destinations Travel smarter Travel tips Travel Tropical Coastal Management Tulamben Tullum Tulum. Tuna Tunnelling Turks and Caicos Turtles Tweezers Ultrsound Umkomaas Unconscious diver Unconsciousness Underground waterways Underground work Underseaa world Underwaater Photos Underwater Photography Underwater Photograper Underwater Photograpgraphy Underwater Photographer Manirelife Underwater Photographers Underwater Research Underwater camera Underwater critters Underwater floral Gardens Underwater hockey Underwater imaging Underwater lights Underwater models Underwater photographer Underwater photography Underwater photos Underwater pho Underwater sound Underwatercommunications Underwater Underwtaer photography University of Stellenbosch Unresponsive Urchins Urinary retention. VGE Vacations Vaccines Vagus nerve Valsalva manoeuvers Valve stem seals Vape Vaping Vasopressors Vasvagal Syncope Venous gas emboli Ventilate Venting Verna van Schak Veterinarian Videography Vincenzo Ferri. Virus infections Volatile fuels WWII wrecks War stories Washout treatments Wastewater Watchman device Water Resistance Water Weakness Weigang Xu Weightloss Weights West Papua Western Cape Diving Wet Lenses Wet diving bell Wetsuit fitting Wetsuites Wetsuits Wetsuit White balance Whitetpped Sharks Wide Angle Photos Wide angles Wildlife park Wildlife Winter Wits Underwater Club Wolf Eels Woman and diving Woman in diving Woman's Health Womans health Woman Women In Diving SA Women and Diving Women in Freediving Women in diving Womens Month Womens health Work of Breathing Workout World Deeepst Dive Record World Records Wound dressings Wreck History Wreck divers Wreck dive Wreck diving Wreck exploration Wreckdiving Wrecks Wrist technology Yachts Yoga Youth diver Zandile Ndholvu Zanzibar Zoology Zooplankton \ Blennies abrasion absolute pressure acoustic neuroma excision adverse seas air-cushioned alert diver altitude alveolar walls anemia antibiotics anticoagulants antiseptics bandages barodontalgia bent-over barbell rows bioassays biodiversity bloodcells blue carbon body art breathing air calories burn canal blockage cannabis oil carbon dioxide toxicity cardiovascular career developments cerebrospinal fluid cervical spine checklist chemo port children child chronic obstructive pulmonary disease clearances closed circuit scuba compressed gass coral growth corrective lenses crystalweed currents cuts cylinder filling daggaolie dagga dead lift decompression algorithms decongestants decongestion deep dive training dehydration discovery dive clubs dive injuries dive medicing dive ready child dive reflex dive tribe divecomputers diver in distress diver rescue diver training dive diving attraction diving hoods doctors dolphns domestic travel dri-suits drowning dry mucous membranes dry suits dry e-cigarettes ear spaces earplugs ears elearning electrolyte imbalance electroytes emergency action plans emergency assessment emergency training environmentally friendly equalising equalizing exposure injuries eyes fEMAL DIVERS fire rescue fish watching fitness Balance fitnes flexible tubing frediving freedivers gas bubble gas poisoning gastric acid gene expression health heartburn heron bridge histidine hospital humidity hypobaric hypoxia immersion and bubble formation immersion pulmonary edema (IPE informal education isopropyl alcohol jaundice join DAN knee lanyard laparoscopic surgery life jackets longevity lower stress lox oxygen level lungs malaise mamalian effect marielife marine pathogens medical Questions medical issues medical procedures medical risk assesment medications mental challenge mental preparedness micro-organisims micro minor illness mucous membranes multilineage dysplasia myelodysplasia nasal steroids nasal near drowning nematocysts neurological newdivers nitrogen bubbles no tanx off-gassed operating theatre operations orthopeadic otitis media out planting outgas pain parameters perforation phillippines phrenic nerve physical challenges pinched nerves plasters pneumoperitoneum polyester-TPU polyether-TPU post dive posture prescription mask preserve prevention professional emergency responders proper equalization psychoactive pulmonary barotrauma. pulmonary injury. pulmunary barotrauma pure oxygen radiation rebreather mask rebreathers retinal detachment risk areas safety stops saturation scissors scuba equipment scuba sea goddesses single use sinus infections situationalawarenes smoking snorkeling. spearfishing spliff sterilising stings strength sub-aquatic sunscreen lotion swimmer's ear swimmer\'s ear swimmers ears tattoo care tecnical diver thermal protection tissue damage toxicity trachea training travel Insurance trimix tympanic membrane unified standards upcycled upwelling virtual coach vision impaired vomiting warmers water quality zinc oxide